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	Provedor de dados BJID (3) BJMBR (1) Autor Barreto,Magali Gonçalves Muniz (1) Boldo-León,X.M. (1) Calore,E.E (1) Calore,N.M.P. (1) Cavaliere,M.J. (1) González-Castro,T.B. (1) Gonçalves,Marilda de Souza (1) Hernández-Díaz,Y. (1) Luna-Arias,J.P. (1) López-López,P. (1) Macedo,Heloisa Werneck de (1) Martínez-López,M.C. (1) Peralta,José Mauro (1) Peralta,Regina Helena Saramago (1) Santos,Fred Luciano Neves (1) Santos,Helena Lúcia Carneiro (1) Soares,Neci Matos (1) Tovilla-Zárate,C.A. (1) Mais... Palavra-chave Amebiasis (4) Entamoeba histolytica (2) Adh112 gene (1) DGGE (1) Diagnosis (1) Diagnostic (1) Fallopian tube (1) Female genital tract (1) Female genital tract infection (1) Multiplex-PCR (1) Neglected diseases (1) PCR (1) Parasites (1) Prevalence (1) Salpingitis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2017 (1) 2011 (1) 2007 (1) 2002 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Comparison of multiplex-PCR and antigen detection for differential diagnosis of Entamoeba histolytica BJID Santos,Helena Lúcia Carneiro; Peralta,Regina Helena Saramago; Macedo,Heloisa Werneck de; Barreto,Magali Gonçalves Muniz; Peralta,José Mauro. Amebiasis is an infection caused by Entamoeba histolytica. However, differentiation between E. histolytica and Entamoeba dispar, which are morphologically identical species, is essential for treatment decision, precaution of the invasive disease and public health. The purpose of the present study was to evaluate a Multiplex -PCR for detection and differentiation of E. histolytica from E. dispar from fresh stool samples in comparison with the coproantigen commercial ELISA. Microscopic examination of stools using the Coprotest method, detection of stool antigen by enzyme-linked immunosorbent assay kit and a home made Multiplex-PCR, were used for the diagnosis of amoebiasis infection. Analysis of the 127 stools samples by microscopy examination demonstrated... Tipo: Info:eu-repo/semantics/article Palavras-chave: Multiplex-PCR; Entamoeba histolytica; Amebiasis. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300013 Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis BJMBR López-López,P.; Martínez-López,M.C.; Boldo-León,X.M.; Hernández-Díaz,Y.; González-Castro,T.B.; Tovilla-Zárate,C.A.; Luna-Arias,J.P.. Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test. The study involved 62 stool samples that were collected from individuals with complaints of gastrointestinal discomfort. Of the 62 samples, 10 (16.1%) were positive for E. histolytica while 52 (83.9%) were negative. No sample was positive for E.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Amebiasis; Neglected diseases; Diagnostic; PCR; DGGE; Adh112 gene. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400607 Salpingitis due to Entamoeba histolytica BJID Calore,E.E; Calore,N.M.P.; Cavaliere,M.J.. We describe the pathology of a unique case of Fallopian tube amebiasis, associated with hydrosalpinx, in a 21-year-old woman. She complained of lower abdominal pain, had a foul-smelling green vaginal discharge and fever during one week. There was a discrete increase in body temperature and a painful abdominal palpation at the lower right side, with signs of local peritoneal irritation. Pathological examination showed a marked dilatation of the fallopian tube and hydrosalpinx. Microscopic examination showed a poorly formed granuloma composed of large macrophages with many Entamoeba histolytica trophozoites inside the fallopian tube. Even though it is a rare disease the correct diagnosis of female genital tract amebiasis is of great importance for the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Female genital tract; Amebiasis; Parasites; Fallopian tube; Female genital tract infection; Salpingitis. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000200007 Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil BJID Santos,Fred Luciano Neves; Gonçalves,Marilda de Souza; Soares,Neci Matos. Amoebiasis is an infection caused by Entamoeba histolytica and is a potential health risk in countries in which health barriers are inappropriate. Since the discovery of Entamoeba dispar, the prevalence of amoebiasis has been modified. OBJECTIVE: This study has standardized the PCR technique applied for the diagnosis of different species of the E. histolytica/E. dispar complex and has evaluated the prevalence of infection among patients attending private and public clinical laboratories in Salvador City, Bahia State, Brazil. RESULTS: Analysis of 52,704 stool samples by microscopic examination demonstrated that 1,788 (3.4%) were positive for the E. histolytica/E. dispar complex and infection occurred more often in samples originated from public clinical... Tipo: Info:eu-repo/semantics/article Palavras-chave: Amebiasis; Entamoeba histolytica; Diagnosis; Prevalence. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200005 Registros recuperados: 4 Primeira ... 1 ... Última

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